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The
KONIK HPLC+HRGC K2 opens the
innovative world of multidimensional LC/HPLC+HRGC.
Combined hyphenated techniques are always of potential interest to
Analytical Chemist. In fact HRGC+MS and HPLC+MS are now standard
equipment in most laboratories and hundreds of methods (most official)
rely on these hyphenated techniques. Their analytic power and
convenience are unquestioned. This innovative HRGC+HPLC coupling, that
puts together two proven techniques in synergy, will follow suit!
KONIK’s
HPLC+HRGC K2, an innovative product from an innovative Company!
KONIK-TECH has been developing and manufacturing HRGC and HPLC Systems
with innovative designs and unmatched performances for the last 25
years. Unique cold septum injectors, high precision ovens, pulse free
pumps, multidimension-multivalve systems, multimode autosamplers,
multifunctional mass spectrometers ... are only a few of KONIK-TECH
impressive innovation trackrecord. This newly developed KONIK
HPLC+HRGC K2 System, fully developed and manufactured by
KONIK-TECH, based in Patent No.: US 6,402,947 B1 and
others, is the next natural step.
HPLC-HRGC,
a happy marriage!
The
innovative KONIK-TECH patented TOTAD interface marries in synergy the
separation and fractionation potential of LC and HPLC, to the
separation, selective detection and quantitation of HRGC, opening new
dimensions... pushing forward borders and limits.
Owing
to its unique characteristics and performance the KONIK HPLC+HRGC K2
offers to the Analytical Chemist a powerful high performance tool to
develop innovative methodologies, while handling, with unsurpassed
comfort, the most complex matrixes to reach far lower detection limits
than some official methods... with ease.
KONIK-TECH’s
brake through is paving the road to make equally this innovative
HPLC+HRGC patented coupling, an standard in every R&D and QC
laboratory around the globe. Its unquestionable and unbiased analytic
power is there. It is only up to the imagination of the Professional
Analytical Chemist familiar with chromatographic methods to take full
advantage of it!
The
principle: An innovative well proven technology
The basic problem to overcome in coupling an LC/HPLC to a HRGC is the
selective elimination of the HPLC solvent from the analytes of interest
that can not enter the HRGC column. The TOTAD interface achieves both
objectives simultaneously while maintaining the HRGC column flow
undisturbed.
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Flow
diagram of the patented KONIK TOTAD HPLC+HRGC coupling
interface
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The
flow diagram depicts the TOTAD interface operating principles. This
patented interface allows the trapping of the analytes in the modified
PTV injector of the KONIK HRGC 4000 B at the
chosen temperature where the trap is held while a digitally controlled
continuos flow of Helium maintains the column flow and eliminates the
solvent from the trap. The HRGC 4000 B time functions of its Control
Module automatically sets the logical valve sequence and the KONIK Mass
Flow Controllers accurately regulate the set flows. These can be
adjusted as well as the temperature of the trap to the solvents being
used.
The
potential:
The following two examples of applications demonstrate the Analytical
Power of the innovative HPLC-HRGC K2 System:
The
Benefits:
Better
qualitative results
The Combined KONIK HPLC+HRGC K2 System provides dual retention indexes,
from both the HPLC fraction chosen and the high precision HRGC retention
times. This dual “retention time locking” can routinely provide
unequivocal chemical identities of the compounds eluted. The System
imposes the selectivity derived of a dual column system and their
particular analytical conditions. These combined selectivity’s are
highly unlikely to be matched by two compounds of different chemical
structure with the exception of optical isomers.
Lower
detection limits
As the patented TOTAD interface uses a trapping and enrichment stage,
likewise in Purge and Trap methods, the analytes are concentrated in the
trap lowering their detection limits.
Simplified
sample preparation
The two stages (LC/HPLC fractionation and trapping), prior to the
introduction into the HRGC column, contribute to simplify the sample
preparation and its conditioning. The HPLC column and solvents used can
contribute totally or partially to the sample clean up depending on the
complexity of the matrix. The trap interface will do the rest,
selectively or universally, trapping and desorbing the compounds of
interest.
Full
automation
The KONIK HPLC+HRGC K2 System is totally automated. After a particular
method is developed the KONIK ROBOKROM
Autosampler can inject any samples (in its original matrix or raw
extracts) into the HPLC column. Qualitative and quantitative results can
be automatically obtained after the GC run is completed.
Saves
time and reduce cost
The KONIK HPLC+HRGC K2 is a rugged workhorse, designed to produce
reliable results sample after sample with significant reduction in total
analysis time, with maximum operator’s comfort. Lower RSD are also
obtained that result in better qualitative and quantitative data... at a
push of a button, or a click of the mouse.
Simplified
method adjustment!... And new methods development
In order to explore and explode the full potential of this
innovative technique KONIK-TECH has made sure those method adjustments
and innovative method can be developed easily and comfortably. All
critical parameters like flows and temperatures are digitally
controlled. Traps are easy to change. All parts in the KONIK HPLC+HRGC
K2 are conveniently accessible.
The
KONIK HPLC+HRGC K2 can be directly coupled to the KONIK
MS Q12 adding the power of Mass Spectrometry to the identification
of unknowns, structural confirmation, or improving quantitation and
lowering detection limits via single or multiple ion detection.
Guarantee
sample integrity, improve recovery and quantitation!
The use of LC/HPLC columns to fractionate selectively, out of any sample
matrix, the compounds of interest might result in the elimination of
tedious, cumbersome, costly and less-friendly extraction (e.g. Sohxlet)
and sample preparation methods. Liquid samples (water, viscous or
dissolved samples) can be directly introduced into the HPLC even without
any prior sample preparation step. This guarantee full protection of the
sample integrity while improving quantitation of the analytes.
Use any LC/HPLC columns and solvents
The KONIK HPLC+HRGC K2 handles all type of columns and all types of
solvents. Both HPLC and LC methods using reversed phase (organic phases
and polar solvents) as well as direct phase methods (columns with
silica, alumina and the like and apolar solvents) are equally suitable
to the System.
Use
any trap and trap any analytes
The limit is the Analytical Chemist imagination. All trapping materials
conventionally used in Purge and Trap methods can be used in accordance
to the sample, solvents and analytes of interest.
In
order to push the «new limits», KONIK-TECH is developing proprietary
materials to widen the scope of applications both in the traps and for
the LC/HPLC fractionation steps.
Analyse
any volatile and many non-volatiles in the HRGC
All trapped volatile substances can be easily, reproducibly, and
quantitatively desorbed. Non-volatile substances can be trapped on
suitable absorbents and derivatised under stopped flow conditions and
further eluted to the HRGC column (patent applied for).
Handle
a wide range of volumes of polar or non-polar solvents
Large amounts of solvents namely from a few micro litres to a few ml can
be passed through the interface to capture the analytes. The volume to
be sampled will be mainly limited by the trap capacity. This sample
concentration step lowers detection limits to unforeseen lower levels
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